LOCUS       40924_23729       11493 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB716 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11493)
  AUTHORS   Tanaka Y, Nakamura S, Kawamukai M, Koizumi N, Nakagawa T.
  TITLE     Development of a series of gateway binary vectors possessing a 
            tunicamycin resistance gene as a marker for the transformation of 
            Arabidopsis thaliana
  JOURNAL   Biosci. Biotechnol. Biochem. 75 (4), 804-807 (2011)
  PUBMED    21512216
REFERENCE   2  (bases 1 to 11493)
  AUTHORS   Tanaka Y, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 11493)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11493)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2011"; volume: "75"; issue: "4"; 
            pages: "804-807"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..11493
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    338..462
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        499..529
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             583..1239
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1584..1886
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1930..2054)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2080..2109
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     CDS             2116..2145
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2161..2190
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2197..2226
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      2257..2509
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2543..2559)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2567..2583
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2591..2621)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2636..2657)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(2693..2945)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2983..4278)
                     /codon_start=1
                     /gene="gpt"
                     /product="UDP-N-acetylglucosamine: dolichol phosphate 
                     N-acetylglucosamine-1-P transferase"
                     /label=gpt
                     /protein_id="BAJ61205.1"
                     /translation="MAARKRASSISIPNKPDPSEPNSAPSEQKMTRKTVSASGEEFRLA
                     PPKLGVIFVISTLLCSLYLYLLCFHYKVDNELKRSILINAGLSLVGFFVTLKMIPVTAR
                     YVLRRNMFGFDINKRGTPQGDIKVPESLGIVVGIVFLIVAIIFQYFNFTEDSNWLVEYN
                     AALASICFMILLGFVDDVLDVPWRVKLVLPSFATLPLLMAYAGHTTIVIPKPLVAYIGL
                     EVLNLGRIYKLYMGLLAVFCTNSINIHAGLNGLEIGQTVVIAAAILIHNVMQIGASVDP
                     EYHQAHAFSIFLTQPLMATSLAMLAYNWYPSSVFVGDTYTVFAGMTMAVVGILGHFSET
                     LLIFFLPQVLNLLLSLPQLAGIVKCPRHRLPRYDPATGLLTGTKDGTLVNVYLRLFGPK
                     SEKSLCIHLLVFQALACAFCFILRHFLAGWYK"
     gene            complement(2983..4278)
                     /gene="gpt"
                     /label=gpt
     promoter        complement(4330..4509)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5015..5039)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5560..6348
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6597..7185
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7371..7511)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7855..8049)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8118..9188)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9620..10246)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt cgactctaga gttatcaaca agtttgtaca aaaaagctga
      361 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
      421 gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggcccc tcgaggggcc
      481 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
      541 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      601 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      661 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      721 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      781 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
      841 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      901 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      961 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     1021 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     1081 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     1141 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
     1201 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta
     1261 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     1321 actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag
     1381 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     1441 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     1501 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     1561 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     1621 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     1681 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     1741 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     1801 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     1861 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     1921 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     1981 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     2041 ttgtacaaag tggttgataa cagcgggtta attaacggtg aacaaaagct aatctccgag
     2101 gaagacttga acggtgaaca aaaattaatc tcagaagaag acttgaacgg actcgacggt
     2161 gaacaaaagt tgatttctga agaagatttg aacggtgaac aaaagctaat ctccgaggaa
     2221 gacttgaacg gtagcgctta gagctcgaat ttccccgatc gttcaaacat ttggcaataa
     2281 agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg
     2341 aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt
     2401 tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc
     2461 gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattggtt
     2521 ccggaaccaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     2581 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     2641 tgagctaact cacattaggc tgaattaggc gcgcctattt ctattaattc ccgatctagt
     2701 aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat tttgttttct
     2761 atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct cataaataac
     2821 gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa ttatatgata
     2881 atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa
     2941 cgatcgggga aattcgcctc gagtgacttc tctgattgca gactatttat accaaccagc
     3001 aagaaagtgt cgaagtatga agcagaaggc gcaggctaga gcctggaaaa cgagaagatg
     3061 gatgcaaaga gacttttctg atttaggacc aaacaacctc aagtaaacat tgacgagtgt
     3121 cccatctttt gtgccggtca gtaatccggt agcaggatca taccttggga gacgatggcg
     3181 cggacatttc acaatcccag caagctgagg aagcgataat agaaggttca gaacttgagg
     3241 aagaaagaag atgaggaggg tttcactgaa gtgacccaga ataccaacaa ctgccatagt
     3301 cattccggcg aagactgtgt aagtgtctcc aacaaaaact gaagaaggat accaattgta
     3361 agcaagcatt gccaaggatg tggccatcag cggttgagta agaaatatcg agaaggcatg
     3421 agcttggtga tactcgggat caacagatgc tccgatttgc ataacgttgt gtatcaggat
     3481 agcagcggca ataacaactg tttgaccgat ttcaaggcca ttcagaccag cgtggatgtt
     3541 aatagagttt gtacaaaaga cagcaagtag ccccatatat aacttatata tccttcctag
     3601 attaagcact tccaagccaa tataagcaac aagaggtttt ggtataacaa tagttgtatg
     3661 accagcataa gccatcaaaa gtggaagggt agcaaaagat ggcaggacaa gttttactct
     3721 ccaaggcaca tcaaggacat catctacaaa tccaagcaaa atcatgaagc aaatagatgc
     3781 tagtgctgca ttatactcca caagccaatt agaatcttca gtgaagttaa aatactgaaa
     3841 tattatcgcc acgatcaaaa agacaatacc aacaacaata cccaatgact ctggcacttt
     3901 aatatctcct tgaggcgtgc ctcttttgtt gatatcaaaa ccaaacatgt tacgtctcag
     3961 aacgtatctg gcagtaacag gaatcatttt gagagtgacg aaaaacccaa ccaagctaag
     4021 ccccgcgtta attagaatcg atcgcttgag ttcattatca accttgtagt gaaaacagag
     4081 aaggtataga tagagcgaac agagaagagt agagatcacg aagatcactc ctaatttcgg
     4141 cggcgcgaga cgaaattcct caccggaagc agaaaccgtc tttcgggtca tcttctgctc
     4201 cgatggcgct gagttgggtt ccgacgggtc cggtttgttg ggaattgaga tcgatgaagc
     4261 tctcttacga gctgccatat cttattgccc cccgggatca gatctggatt gagagtgaat
     4321 atgagactct aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca
     4381 agaaatattt gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc
     4441 tgacgtatgt gcttagctca ttaaactcca gaaacccgcg gctgagtggc tccttcaacg
     4501 ttgcggttct gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat
     4561 aacgtgactc ccttaattct ccgctcatga tcttgatccc ctgcgccatc agatccttgg
     4621 cggcaagaaa gccatccagt ttactttgca gggcttccca accttaccag agggcgcccc
     4681 agctggcaat tccggttcgc ttgctgtcca taaaaccgcc cagtctagct atcgccatgt
     4741 aagcccactg caagctacct gctttctctt tgcgcttgcg ttttcccttg tccagatagc
     4801 ccagtagctg acattcatcc ggggtcagca ccgtttctgc ggactggctt tctacgtgtt
     4861 ccgcttcctt tagcagccct tgcgccctga gtgcttgcgg cagcgtgaag ctgatccgtc
     4921 gagattaata gaaataaatt cagcctaatt cggcgttaat tcagtacatt aaaaacgtcc
     4981 gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat atcctgccac
     5041 cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc gatacaggca
     5101 gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag actttgctca
     5161 tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa cacggatgat
     5221 ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct gtgatcaatt
     5281 cgggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt
     5341 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg
     5401 gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc
     5461 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
     5521 acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc
     5581 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg
     5641 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat
     5701 attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc
     5761 aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa
     5821 gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg
     5881 caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc
     5941 gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt
     6001 ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat
     6061 gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg
     6121 cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc
     6181 gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct
     6241 agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa
     6301 gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctagct
     6361 agaaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac
     6421 taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag
     6481 cgtgcataat aagccctaca caaattggga gatatatcat gcatgaccaa aatcccttaa
     6541 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6601 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6661 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6721 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     6781 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6841 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6901 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6961 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7021 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7081 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7141 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7201 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     7261 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     7321 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     7381 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     7441 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
     7501 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     7561 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     7621 tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg
     7681 agtggcgacg gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt
     7741 tttgagcggc cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc
     7801 gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca
     7861 caggccaggc gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg
     7921 ccttttttct cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt
     7981 gggttcccaa tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg
     8041 ctatccacag gaaagagacc ttttcgacct ttttcccctg ctagggcaat ttgccctagc
     8101 atctgctccg tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc
     8161 atgactagga tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca
     8221 tttgacccga tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca
     8281 ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt
     8341 gccaggcggt agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc
     8401 gtcagcacgt ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg
     8461 atctcgatgt actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag
     8521 gcttcaccct cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg
     8581 gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg
     8641 ccatcggctc gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg
     8701 ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc
     8761 atcagtacca ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct
     8821 acgtgcccgt ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac
     8881 agacggaaaa cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc
     8941 atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca
     9001 ccggctgccg gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat
     9061 tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac
     9121 ttctccacca ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga
     9181 ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcagacaa
     9241 cccagccgct tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt
     9301 cggtgcctgg ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac
     9361 tcatttattc atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg
     9421 gtaatggtct tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc
     9481 aactgaaagt tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc
     9541 ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc
     9601 tctttacctc attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct
     9661 cgcgggcagc gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc
     9721 ctgggtagct cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca
     9781 gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg
     9841 ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg
     9901 cggtggccca tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct
     9961 tgatcgcgga cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc
    10021 gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta
    10081 gcggttgatc ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga
    10141 ctaacagaac atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg
    10201 tcttgcctga cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg
    10261 tatttgttta tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta
    10321 ctcaaataca catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc
    10381 cggccaggcc gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt
    10441 tgagacgtgc gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc
    10501 ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct
    10561 tggcgttcat tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag
    10621 gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac
    10681 agggtcgagc gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg
    10741 tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac
    10801 ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa
    10861 cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg
    10921 gtgtcggccc acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg
    10981 gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca
    11041 acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg
    11101 ccggtgatct tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg
    11161 ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg
    11221 ctcttgttca tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta
    11281 aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag
    11341 gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca
    11401 ctatagcagc ggaggggttg gatcaaagta ctttgatccc gaggggaacc ctgtggttgg
    11461 catgcacata caaatggacg aacggataaa cct
//
